Product Description

Catalog number: E-EL-H***9
Size: *6T
Reactivity: Human
Detection Range: *9.*6~***0 pg/mL
Sensitivity: *3.*4 pg/mL
Lead time: 7~*0days 

Intended use
This ELISA kit can be applied to the in vitro quantitative determination of Human RNASE3/ECP concentrations in serum, plasma and other biological fluids.

Test principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RNASE3/ECP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RNASE3/ECP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RNASE3/ECP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of **0 nm ± 2 nm. The OD value is proportional to the concentration of Human RNASE3/ECP. The concentration of Human RNASE3/ECP in samples can be calculated by comparing the OD of the samples to the standard curve.